Hydrostatic pressure induced Dirac semimetal in black phosphorus

Motivated by recent experimental observation of an hydrostatic pressure induced transition from semiconductor to semimetal in black phosphorus [Chen et al. in arXiv:1504.00125], we present the first principles calculation on the pressure effect of the electronic structures of black phosphorus. It is found that the band crossover and reversal at the Z point occur around the critical pressure Pc1=1.23 Gpa, and the band inversion evolves into 4 twofold-degenerate Dirac cones around the Z point, suggesting a 3D Dirac semimetal. With further increasing pressure the Dirac cones in the Gamma-Z line move toward the Gamma point and evolve into two hole-type Fermi pockets, and those in the Z-M lines move toward the M point and evolve into 2 hole-type Fermi pockets up to P=4.0 Gpa. It demonstrates clearly that the Lifshitz transition occurs at $P_{c1}$ from semiconductor to 3D Dirac semimetal protected by the nonsymmorphic space symmetry of bulk. This suggests the bright perspective of black phosphorus for optoelectronic and electronic devices due to its easy modulation by pressure.Comment: 7 pages, 9 figures, and 2 table

Double-stranded break can be repaired by single-stranded oligonucleotides via the ATM/ATR pathway in mammalian cells

Single-stranded oligonucleotide (SSO)-mediated gene modification is a newly developed tool for site-specific gene repair in mammalian cells; however, the corrected cells always show G2/M arrest and cannot divide to form colonies. This phenomenon and the unclear mechanism seriously challenge the future application of this technique. In this study, we developed an efficient SSO-mediated DNA repair system based on double-stranded break (DSB) induction. We generated a mutant EGFP gene with insertions of 24 bp to 1.6 kb in length as a reporter integrated in mammalian cell lines. SSOs were successfully used to delete the insertion fragments upon DSB induction at a site near the insertion. We demonstrated that this process is dependent on the ATM/ATR pathway. Importantly, repaired cell clones were viable. Effects of deletion length, SSO length, strand bias, and SSO modification on gene repair frequency were also investigated. © 2008 Mary Ann Liebert, Inc.published_or_final_versio

Iron-Catalyzed Nitrene Insertion Reaction for Facile Construction of Amide Compounds

A facile method for the construction of amide compounds from aldehydes by an iron-catalyzed nitrene insertion reaction has been developed. Both aryl and aliphatic aldehydes can directly afford the corresponding amides with an iron(II)-terpyridine (tpy) complex formed in situ as catalyst, and PhI=NTs as nitrogen source under mild reaction conditions. An ESI-MS study revealed the formation of [Fe(tpy)NTs)]+ as a reaction intermediate. © Georg Thieme Verlag Stuttgart - New York.postprin

[FeIII(TF4DMAP)OTf] catalysed anti-Markovnikov oxidation of terminal aryl alkenes to aldehydes and transformation of methyl aryl tertiary amines to formamides with H2O2 as a terminal oxidant

Anti-Markovnikov oxidation of terminal aryl alkenes to aldehydes and transformation of N-methyl aryl tertiary amines to formamides with H2O2 as a terminal oxidant under mild conditions have been achieved with moderate to good product yields using [FeIII(TF4DMAP)OTf] as catalyst.postprin

An emigration genetic algorithm and its application to multiobjective optimal designs of electromagnetic devices

Author name used in this publication: S. L. Ho2003-2004 > Academic research: refereed > Publication in refereed journalVersion of RecordPublishe

Synergistic Effect of Chlorogenic Acid and Caffeic Acid with Fosfomycin on Growth Inhibition of a Resistant Listeria monocytogenes Strain

Listeria monocytogenes, a human foodborne pathogen that causes listeriosis with high-rate mortality, has been reported to be resistant to commonly used antibiotics. New antibiotics or cocktails of existing antibiotics with synergistic compounds are in high demand for treating this multi-drug-resistant pathogen. Fosfomycin is one of the novel and promising therapeutic antibiotics for the treatment of listeriosis. However, some L. monocytogenes strains with the FosX gene were recently reported to survive from the fosfomycin treatment. This work aims to identify FosX inhibitors that can revive fosfomycin in treating resistant L. monocytogenes. Since structures and activities of the FosX protein in L. monocytogenes have been well studied, we used an integrated computational and experimental approach to identify FosX inhibitors that show synergistic effect with fosfomycin in treating resistant L. monocytogenes. Specifically, automated ligand docking was implemented to perform virtual screening of the Indofine natural-product database and FDA-approved drugs to identify potential inhibitors. An in vitro bacterial growth inhibition test was then utilized to verify the effectiveness of identified compounds combined with fosfomycin in inhibiting the resistant L. monocytogenes strains. Two phenolic acids, i.e., caffeic acid and chlorogenic acid, were predicted as high-affinity FosX inhibitors from the ligand-docking platform. Experiments with these compounds indicated that the cocktail of either caffeic acid (1.5 mg/mL) or chlorogenic acid (3 mg/mL) with fosfomycin (50 mg/L) was able to significantly inhibit the growth of the pathogen. The finding of this work implies that the combination of fosfomycin with either caffeic acid or chlorogenic acid is of potential to be used in the clinical treatment of Listeria infections

Adipose-derived stromal cells protect intervertebral disc cells in compression: implications for stem cell regenerative disc therapy

INTRODUCTION: Abnormal biomechanics plays a role in intervertebral disc degeneration. Adipose-derived stromal cells (ADSCs) have been implicated in disc integrity; however, their role in the setting of mechanical stimuli upon the disc's nucleus pulposus (NP) remains unknown. As such, the present study aimed to evaluate the influence of ADSCs upon NP cells in compressive load culture. METHODS: Human NP cells were cultured in compressive load at 3.0MPa for 48 hours with or without ADSCs co-culture (the ratio was 50:50). We used flow cytometry, live/dead staining and scanning electron microscopy (SEM) to evaluate cell death, and determined the expression of specific apoptotic pathways by characterizing the expression of activated caspases-3, -8 and -9. We further used real-time (RT-) PCR and immunostaining to determine the expression of the extracellular matrix (ECM), mediators of matrix degradation (e.g. MMPs, TIMPs and ADAMTSs), pro-inflammatory factors and NP cell phenotype markers. RESULTS: ADSCs inhibited human NP cell apoptosis via suppression of activated caspase-9 and caspase-3. Furthermore, ADSCs protected NP cells from the degradative effects of compressive load by significantly up-regulating the expression of ECM genes (SOX9, COL2A1 and ACAN), tissue inhibitors of metalloproteinases (TIMPs) genes (TIMP-1 and TIMP-2) and cytokeratin 8 (CK8) protein expression. Alternatively, ADSCs showed protective effect by inhibiting compressive load mediated increase of matrix metalloproteinases (MMPs; MMP-3 and MMP-13), disintegrin and metalloproteinase with thrombospondin motifs (ADAMTSs; ADAMTS-1 and 5), and pro-inflammatory factors (IL-1beta, IL-6, TGF-beta1 and TNF-alpha). CONCLUSIONS: Our study is the first in vitro study assessing the impact of ADSCs on NP cells in an un-physiological mechanical stimulation culture environment. Our study noted that ADSCs protect compressive load induced NP cell death and degradation by inhibition of activated caspase-9 and -3 activity; regulating ECM and modulator genes, suppressing pro-inflammatory factors and preserving CK8. Consequently, the protective impact of ADSCs found in this study provides an essential understanding and expands our knowledge as to the utility of ADSCs therapy for intervertebral disc regeneration.published_or_final_versio

An implementation of synthetic generation of wind data series

Wind power fluctuation is a major concern of large scale wind power grid integration. To test methods proposed for wind power grid integration, a large amount of wind data with time series are necessary and will be helpful to improve the methods. Meanwhile, due to the short operation history of most wind farms as well as limitations of data collections, the data obtained from wind farms could not satisfy the needs of data analysis. Consequently, synthetic generation of wind data series could be one of the effective solutions for this issue. In this paper, a method is presented for generating wind data series using Markov chain. Due to the high order Markov chain, the possibility matrix designed for a wind farm could cost a lot of memory, which is a problem with current computer technologies. Dynamic list will be introduced in this paper to reduce the memory required. Communication errors are un-avoidable on long way signal transmission between the control centre and wind farms. Missing of data always happens in the historical wind data series. Using these data to generate wind data series may result in some mistakes when searching related elements in the probability matrix. An adaptive method will be applied in this paper to solve the problem. The proposed method will be verified using a set of one-year historical data. The results show that the method could generate wind data series in an effective way. © 2013 IEEE.published_or_final_versio

Simultaneous determination of flavonoids and triterpenoids in Cyclocarya paliurus leaves using high-performance liquid chromatography

Background: Cyclocarya paliurus is an endangered plant and endemic to China. The leaves of C. paliurus have been used in drug formulations and as ingredients in functional foods in China. The aim of this study was to develop an effective method to extract most of the compounds and to establish a simplified HPLC analytical method to determine the contents of major bioactive compounds simultaneously.Materials and methods: High-performance liquid chromatography (HPLC) coupled with a photodiode array detector (PDA) was used for the simultaneous determination of the major flavonoids and triterpenoids in C. paliurus leaves.Results: Ultrasonic extraction in 100% methanol for 30 min was adopted as the optimal extraction method for C. paliurus leaves. The separation conditions were optimized using a Phenomenex C18 ODS column (250 mm × 4.6 mm, 5 μm) with a mobile phase of acetonitrile and 0.02% formic acid and a detection wavelength of 205 nm. The validation data indicated that this new HPLC analytical method successfully quantified the provenance and seasonal variations of seven major compounds (three flavonoids and four triterpenoids) in C. paliurus leaves.Conclusion: The study provided a novel and simplified approach to simultaneously determine the quantity of major flavonoids and triterpenoids in C. paliurus leaves. The results could promote the optimization of silvicultural systems for quality control of C. paliurus.Key words: Cyclocarya paliurus; HPLC; flavonoids; triterpenoid

Characterization of implementation limits and identification of optimization strategies for sustainable water resource recovery through life cycle impact analysis

This is the final version. Available from Elsevier via the DOI in this record.How we manage alternative freshwater resources to close the gap between water supply and demand is pivotal to the future of the environment and human well-being. Increased scarcity of water for agricultural irrigation in semi-arid and arid regions has resulted in a growing interest in water reuse practices. However, insight into the life cycle impacts and potential trade-offs of these emerging practices are still limited by the paucity of systematic evaluations of different water reuse implementations. In this study, a host of environmental and human health impacts at three implementation levels of allowing water reclamation for crop irrigation was comparatively evaluated across the operational landscape via a combination of scenario modelling, life-cycle impact analyses and Monte Carlo simulations. Net harvesting of reclaimed water for irrigation was found to be dependent upon the sophistication of the treatment processes, since multistage and complex configurations can cause greater direct water consumption during processing. Further, the direct benefits of water resource recovery can be essentially offset by indirect adverse impacts, such as mineral depletion, global warming, ozone depletion, ecotoxicity, and human health risks, which are associated with increased usage of energy and chemicals for rigorous removal of contaminants, such as heavy metals and contaminants of emerging concern. Nonetheless, expanded simulations suggest the significance of concurrently implementing energy recovery, nutrient recycling, and/or nature-based, chemical-free water technologies to reduce the magnitude of negative impacts from engineered water reclamation processes.Royal Society (Charity)Beijing Talents FoundationBeijing Nova Program, ChinaNational Natural Science Foundation of ChinaYouth Innovation Promotion Association of the Chinese Academy of Science